Cells, Free Full-Text

High-resolution 3D images of organelles are of paramount importance in cellular biology. Although light microscopy and transmission electron microscopy (TEM) have provided the standard for imaging cellular structures, they cannot provide 3D images. However, recent technological advances such as serial block-face scanning electron microscopy (SBF-SEM) and focused ion beam scanning electron microscopy (FIB-SEM) provide the tools to create 3D images for the ultrastructural analysis of organelles. Here, we describe a standardized protocol using the visualization software, Amira, to quantify organelle morphologies in 3D, thereby providing accurate and reproducible measurements of these cellular substructures. We demonstrate applications of SBF-SEM and Amira to quantify mitochondria and endoplasmic reticulum (ER) structures.

Serial Number Alcohol 120 1.9 6 - Colaboratory

Cell-free Fetal DNA — A Trigger for Parturition

Cell-Free Gene Expression: Methods and Protocols

Remote immune processes revealed by immune-derived circulating cell-free DNA

JCM, Free Full-Text

Cell-Free DNA and Apoptosis: How Dead Cells Inform About the Living - ScienceDirect

Antibodies, Free Full-Text

Cell-free Macromolecular Synthesis

Remote immune processes revealed by immune-derived circulating cell-free DNA

Cell-Free Protein Expression

Cancer type classification using plasma cell-free RNAs derived from human and microbes

Anomalous Scaling of Gene Expression in Confined Cell-Free Reactions