Cells, Free Full-Text
Por um escritor misterioso
Last updated 17 fevereiro 2025
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Olive flounder (Paralichthys olivaceus) muscle satellite cells (OFMCs) were obtained by enzymatic primary cell isolation and the explant method. Enzymatic isolation yielded cells that reached 80% confluence within 8 days, compared to 15 days for the explant method. Optimal OFMC growth was observed in 20% fetal bovine serum at 28 °C with 0.8 mM CaCl2 and the basic fibroblast growth factor (BFGF) to enhance cell growth. OFMCs have become permanent cell lines through the spontaneous immortalization crisis at the 20th passage. Olive flounder skeletal muscle myoblasts were induced into a mitogen-poor medium containing 2% horse serum for differentiation; they fused to form multinucleate myotubes. The results indicated complete differentiation of myoblasts into myotubes; we also detected the expression of the myogenic regulatory factors myoD, myogenin, and desmin. Upregulation (Myogenin, desmin) and downregulation (MyoD) of muscle regulation factors confirmed the differentiation in OFMCs.
An illustration of the full-duplex cell-free massive MIMO system.
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Serial Number Alcohol 120 1.9 6 - Colaboratory
![Cells, Free Full-Text](https://media.springernature.com/full/springer-static/image/art%3A10.1038%2Fsrep30442/MediaObjects/41598_2016_Article_BFsrep30442_Fig1_HTML.jpg)
Cell-free methods to produce structurally intact mammalian membrane proteins
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Cell-Free DNA and Apoptosis: How Dead Cells Inform About the Living - ScienceDirect
Labile coat: reason for noninfectious cell-free varicella-zoster virus in culture. - Abstract - Europe PMC
![Cells, Free Full-Text](https://www.nejm.org/na101/home/literatum/publisher/mms/journals/content/nejm/2014/nejm_2014.370.issue-26/nejmcibr1404324/20140620/images/img_medium/nejmcibr1404324_f1.jpeg)
Cell-free Fetal DNA — A Trigger for Parturition
![Cells, Free Full-Text](https://ars.els-cdn.com/content/image/1-s2.0-S2666379123001489-fx1.jpg)
Nucleic acid biomarkers of immune response and cell and tissue damage in children with COVID-19 and MIS-C - ScienceDirect
![Cells, Free Full-Text](https://www.researchgate.net/profile/Natalio-Krasnogor/publication/282702033/figure/fig2/AS:286568797093889@1445334733772/Scheme-of-cell-free-cloning-using-DMF-A-The-full-length-construct-generated-by-POP.png)
Scheme of cell free cloning using DMF. ( A ) The full length construct
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Nt Novo Cella Get File - Colaboratory
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Antibodies, Free Full-Text
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IJMS, Free Full-Text
![Cells, Free Full-Text](https://www.frontiersin.org/files/Articles/481280/fmolb-06-00067-HTML/image_m/fmolb-06-00067-g001.jpg)
Frontiers Combining Cell-Free Protein Synthesis and NMR Into a Tool to Study Capsid Assembly Modulation
Cells, Free Full-Text
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