Knockout! A CRISPR/Cas Gene Targeting Lab – miniPCR bio

This lab is easy for teachers to prepare, makes gene targeting accessible to students, and packs a powerful punch! Become a genetic engineer and use CRISPR/Cas to disable a gene in bacteria. Scientists use the CRISPR/Cas system to alter the DNA in living cells. Now…
Perform cutting-edge genome editing! This lab allows advanced students to use the CRISPR/Cas system to disrupt a gene in bacteria and observe the resulting phenotypic change. 

Perform cutting-edge genome editing! This lab allows advanced students to use the CRISPR/Cas system to disrupt a gene in bacteria and observe the

Knockout! A CRISPR/Cas Gene Targeting Lab

Resources: Workshop Report: Summer 2020 Virtual CRISPR in the Classroom

Knockout! A CRISPR/Cas Gene Editing Lab from miniPCR bio™

Reproducibility of CRISPR-Cas9 methods for generation of conditional mouse alleles: a multi-center evaluation, Genome Biology

miniPCR bio on LinkedIn: #crispr #crisprcas9 #crispred

Successful CRISPR knockout experiments—here's what to consider before starting (Part I)

Frontiers CRISPR Toolbox for Genome Editing in Dictyostelium

Cell Free Gene Expression Methods and Protocols Humana, 2022, PDF, Biosensor

Optional follow-up: Confirm gene knockout at the molecular level.

Knockout! PCR Genotyping Experiment

CRISPR/Cas Systems: A New Biomedical and Agricultural Diagnostic Devices for Viral Diseases

Genome-wide CRISPR-Cas9 knockout library screening identified PTPMT1 in cardiolipin synthesis is crucial to survival in hypoxia in liver cancer - ScienceDirect

P51™ QPCR Lab – TGAC

CRISPR/Cas – miniPCR bio