Advanced Quantitative Fluorescence Microscopy to Probe the Molecular Dynamics of Viral Entry, Science Lab

Viral entry into the host cell requires the coordination of many cellular and viral proteins in a precise order. Modern microscopy techniques are now allowing researchers to investigate these interactions with higher spatiotemporal resolution than ever before. Here we present two examples from the field of HIV research that make use of an innovative quantitative imaging approach as well as cutting edge fluorescence lifetime-based confocal microscopy methods to gain novel insights into how HIV fuses to cell membranes and enters the cell.

Identification of SARS-CoV-2 3CL Protease Inhibitors by a Quantitative High-Throughput Screening

Labeling of virus components for advanced, quantitative imaging analyses - Sakin - 2016 - FEBS Letters - Wiley Online Library

Amelioration of SARS-CoV-2 infection by ANO6 phospholipid scramblase inhibition - ScienceDirect

Ultrafast, sensitive, and portable detection of COVID-19 IgG using flexible organic electrochemical transistors

Lighting up the Native Viral RNA Genome with a Fluorogenic Probe for the Live-Cell Visualization of Virus Infection

Influenza A virus ribonucleoproteins form liquid organelles at endoplasmic reticulum exit sites

Quantitative description of a contractile macromolecular machine

J. Imaging, Free Full-Text

Rapid HIV-1 Capsid Interaction Screening Using Fluorescence Fluctuation Spectroscopy

Frontiers Evolution, Interspecies Transmission, and Zoonotic Significance of Animal Coronaviruses

Single molecule imaging simulations with advanced fluorophore photophysics